Informatics Publishing Limited

Jajow Swapna ¹, Chandaka Madhu ¹, Mallepelli Srivani ¹, M. Sumalatha ¹, Y. Nehalatha ¹, Y. Anusha ¹

Affiliations
1 Sri Indu College of Pharmacy, Sheriguda, Hyderabad (A.P)-501 301, IN

Abstract

A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the estimation of Metformin HCl (MET) and Pioglitazone (PIO) in pure and in pharmaceutical dosage forms. A BDS Hypersil C18 column (250x4.6mm, 5μ) was used with a mobile phase containing a mixture of Acetonitrile and Potassium dihydrogen ortho phosphate buffer (pH-3) in the ratio of 50: 50. The flow rate was 1ml/min and effluents were monitored at 238nm and eluted at 2.81min (MET) and 4.57min (PIO). Calibration curve was plotted with a range from 40-240 μg / ml for MET and 12-72 μg / ml for PIO. The assay was validated for the parameters like accuracy, precision, specificity, robustness, ruggedness and system suitability parameters. The proposed method can be useful in the routine analysis for the determination on metformin and pioglitazone in pharmaceutical dosage forms.

Keywords

Metformin Hydrochloride, Pioglitazone Hydrochloride, Reverse Phase Hplc, Pharmaceutical Dosage Forms, Simultaneous Estimation.

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Chandaka Madhu ¹, L. Harish ¹, K. Narsimha Reddy ¹, S. Sagar ¹, D. Soumya ¹, G. Pavan Kumar ¹

Affiliations
1 Sri Indu Institute of Pharmacy, Sheriguda, Ibrahimpatnam, R.R Dist, IN

Abstract

The kidney stone is one of the most widely spreading renal disorders in the world. The present study was undertaken to evaluate the efficacy of Plectranthus tomentora in reducing the growth of calcium oxalate stones in ethylene glycol induced model. Upon administration of Furosemide (20mg/kg), aqueous extract of ischolar_mains and seeds of Plectranthus tomentora (500 and 1000 mg/kg) on hyperoxaluria rats shows the significant activity in decrease kidney stones and serum levels (calcium, phosphorous, creatinine, urea) both not that as standard drug Furosemide.

Keywords

Plectranthus tomentora, Calcium Oxalate, Ethylene Glycol, Furosemide.

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Chandaka Madhu ¹, K. Hindu ¹, C. D. Sudeepthi ¹, P. Maneela ¹, K. Venkat Reddy ¹, B. Bhagya Sree ¹

Affiliations
1 Sri Indu Institute of Pharmacy, Ibrahimpatnam, Hyderabad, R.R Dist, IN

Abstract

The anti-ulcer activity of aqueous extract of Aegle marmelos leaves was investigated on a indomethacin induced ulcer models in wistar rats. In model the common parameter determined was ulcer-index. aqueous extract of dosage 175, 350 mg/kg p.o produced significant inhibition of gastric lesions induced by indomethacin induced ulcers. The extract 175mg/kg & 350mg/kg showed significant (p<0.01) reduction in gastric volume, free acidity and ulcer index as compared to control. This present study indicate that Aegle marmelos leaves extract have potential anti ulcer activity. This results may further suggests that aqueous extract was found to posses antiulcerogenic as well as ulcer healing property, which might be anti secretary activity.

Keywords

Anti-Ulcer, Aegle marmelos, Antisecretory, Indomethacin.

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